Selective inhibition by sorbylhydroxamic acid of deoxyribonucleic acid synthesis in Ehrlich ascites tumor cells.
نویسندگان
چکیده
Sorbylhydroxamic acid (SOHA) inhibited the incorporation in vitro of thymidine into deoxyribonucleic acid in Ehrlich ascites tumor cells, with no appreciable effect on the incorporation of uridine into ribonucleic acid or of L-leucine into protein. Hexanoylhydroxamic acid, devoid of the conjugated double-bond system, had no such activity. The concentration of SOHA conferring 50% inhibition of DNA synthesis was approximately 6 X IGT4 M. Inhibition was
منابع مشابه
Selective Inhibition by Sorbylhydroxamic Acid of Deoxyribonucleic Acid Synthesis in Ehrlich Ascites Tumor Cells1
Sorbylhydroxamic acid (SOHA) inhibited the incorporation in vitro of thymidine into deoxyribonucleic acid in Ehrlich ascites tumor cells, with no appreciable effect on the incorporation of uridine into ribonucleic acid or of L-leucine into protein. Hexanoylhydroxamic acid, devoid of the conjugated double-bond system, had no such activity. The concentration of SOHA conferring 50% inhibition of D...
متن کاملOn the mechanism of inhibition of deoxyribonucleic acid synthesis in Ehrlich ascites tumor cells by deoxyadenosine in vitro.
It has previously been shown that the incorporation qf both C14-formate into DNA1 thymine and P32-orthophosphate into DNA phosphate of the hypotetraploid strain of Ehrlich ascites tumor cells in vitro is strongly inhibited by deoxyadenosine.2 It was, furthermore, shown that this inhibition is not due to a prevention of the de novo synthesis of acid-soluble thymine compounds. The incorporation o...
متن کاملNucleic acid metabolism in Ehrlich ascites tumor cells.
The incorporation of P32 into deoxyribonucleic acid (DNA) by Ehrlich ascites tumor cells has been found to be considerably more radioresistant when carried out in vitro than in vivo (6). To study the nature of the in vitro incorporation and explore possible reasons for its radioresistance, we have in vestigated the incorporation of P32 and various C'Mabeled precursors into the nucleic acids of ...
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The loosely bound chromatin proteins of Ehrlich ascites hyperdiploid cells have been prepared by extraction of chromatin with 0.35 M NaCl. Sodium dodecyl sulfate gel electrophoresis of the 0.35 M NaCl-soluble chromatin proteins reveals high heterogeneity with a molecular weight range of 10,000 to 170,000. The 0.35 M NaCl-soluble chromatin proteins contain many components similar to the more tig...
متن کاملMetabolic studies on 6-methylaminopurine.
and in mice bearing Ehrlich ascites tumor has been investigated. The results indicate that the exogenous compound is utilized equally well for the synthesis of both adenine and guanine of deoxyribonucleic acid and ribonucleic acid. No evidence for the incor poration of intact 6-methylaminopurine into the nucleic acids of liver, kidney, spleen, intestine, or tumor cells was obtained. The princip...
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ورودعنوان ژورنال:
- Cancer research
دوره 30 1 شماره
صفحات -
تاریخ انتشار 1970